HCA Data Explorer

Single-cell RNA-seq reveals heterogeneity within human pre-cDCs

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Updated January 19, 2022

Here, we use a microfluidics-based approach to prepare single-cell RNA-Seq libraries from 164 primary human conventional dendritic cells (cDCs) as well as cord blood (452) and blood (341) pre-cDCs. We examined heterogeneity between individual cells to document potential subpopulations within human cDCs and pre-cDCs.

Thiago Y OliveiraThe Rockefeller Universitytoliveira@rockefeller.edu
Thiago Y Oliveira (Experimental Scientist)1
Michel C Nussenzweig (Principal Investigator)1
1The Rockefeller University
Parisa Nejad
Kamron Mojabe
Beagan Nguy

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/05be4f37-4506-429b-b112-506444507d62

Supplementary links are provided by contributors and represent items such as additional data which can’t be hosted here; code that was used to analyze this data; or tools and visualizations associated with this specific dataset.

1.ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE89nnn/GSE89232/suppl/GSE89232_expMatrix.txt.gz
INSDC Project Accessions:GEO Series Accessions:

Atlas

None

Analysis Portals

None

Project Label

HumanDCsFromPre-cDCs

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

blood

Organ Part

2 organ parts

Selected Cell Types

2 cell types

Disease Status (Specimen)

Unspecified

Disease Status (Donor)

normal

Development Stage

human adult stage

Library Construction Method

Smart-seq2

Nucleic Acid Source

single cell

Paired End

false

Analysis Protocol

MultiSampleSmartSeq2_v2.2.6, SmartSeq2SingleSample_v5.1.5

File Format

5 file formats

Cell Count Estimate

957

Donor Count

2
bai1,543 file(s)bam1,543 file(s)fastq.gz1,543 file(s)loom1 file(s)txt.gz1 file(s)