HCA Data Explorer

Single-cell RNA-seq in monozygotic twins discordant for multiple sclerosis

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Updated September 30, 2021

Here we investigated the earliest possible evidence of subclinical neuro-inflammation (SCNI) using a small cohort of monozygotic twins where one sibling had clinically definite MS and the other been clinically "healthy" but has a maximal genetic for developing MS. In contrast to subjects with radiologically isolated syndrome (RIS), our group of very early SCNI does not even fulfill the (arbitrary) MRI criteria for RIS but have more subtle MRI changes and/or evidence of neuro-inflammation in the CSF, e.g. oligoclonal bands (OCBs). For analyzing CSF samples from Twin pairs and controls in greater detail we applied single-cell whole transcriptome sequencing (scRNAseq). Our findings demonstrate that even the earliest experimentally approachable stage of MS is characterized by synergistic activation of CD8+ T cells, CD4+ T cells and B cells.

Eduardo BeltránInstitute of Clinical Neuroimmunology, LMU MunichEduardo.Beltran@med.uni-muenchen.de
Eduardo Beltrán (Experimental Scientist)1
1Institute of Clinical Neuroimmunology, LMU Munich
Kamron Mojabe
Gavin James Drumm
Parisa Nejad

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/0c09fade-e079-4fde-8e60-6725b8c1d84b

Supplementary links are provided by contributors and represent items such as additional data which can’t be hosted here; code that was used to analyze this data; or tools and visualizations associated with this specific dataset.

1.ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE127nnn/GSE127969/suppl/GSE127969_cellID_barcode_SRR.txt.gz,ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE127nnn/GSE127969/suppl/GSE127969_counts_TPM_ALL.csv.gz,ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE127nnn/GSE127969/suppl/GSE127969_sc_cell_info.txt.gz
INSDC Project Accessions:GEO Series Accessions:INSDC Study Accessions:

Analysis Portals

None

Project Label

CsfFromTwinsDiscordantForMultSclerosis

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

Immune system

Organ Part

cerebrospinal fluid

Selected Cell Types

7 cell types

Disease Status (Specimen)

6 disease statuses

Disease Status (Donor)

6 disease statuses

Development Stage

human adult stage

Library Construction Method

Smart-like

Nucleic Acid Source

single cell

Paired End

true

File Format

3 file formats

Cell Count Estimate

3.4k

Donor Count

16
csv.gz1 file(s)fastq.gz60 file(s)txt.gz2 file(s)