Single cell RNA-seq of HeLa CCL2
We measured 720 HeLa-CCL2 cells (288 from the 9th passage, 288 from the 14th passage and 144 from the 20th passage) in this work on an Illumina HiSeq 4000 platform (sequenced by Novogene, China) for 150 bp paired-end sequencing. The protocol was based on Tang’s modification on SMART-Seq2. Single cells were captured by mouth pipette under the stereomicroscope(Nikon SZM745)and followed by the protocol. Every cell was assigned with 250 M raw data on average.
To reference this project, please use the following link:
Downloaded and exported data is governed by the HCA Data Release Policy and licensed under the Creative Commons Attribution 4.0 International License (CC BY 4.0). For more information please see our Data Use Agreement.
Atlas
Analysis Portals
NoneProject Label
scRNAofHelaCCL2Species
Homo sapiens
Sample Type
cellLines
Anatomical Entity
uterus
Organ Part
cervix
Selected Cell Types
epithelial cell of cervix
Model Organ
uterus
Disease Status (Specimen)
adenocarcinoma
Disease Status (Donor)
adenocarcinoma
Development Stage
human adult stage
Library Construction Method
Smart-like
Nucleic Acid Source
single cell
Paired End
trueAnalysis Protocol
analysis_protocol_1File Format
Cell Count Estimate
720Donor Count
1