HCA Data Explorer

Population-scale single-cell RNA-seq profiling across dopaminergic neuron differentiation.

Access Granted
Updated July 19, 2022

An efficient pooling strategy was used to differentiate 215 iPS cell lines towards a midbrain neural fate, including dopaminergic neurons. Over 1 million cells were sampled across three differentiation timepoints and profiled using single cell RNA sequencing.

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J Jerber1
D D Seaton1
A SE Cuomo1
N Kumasaka1
J Haldane1
J Steer1
M Patel1
D Pearce1
M Andersson1
M J Bonder1
E Mountjoy1
M Ghoussaini1
M A Lancaster1
J C Marioni1
F T Merkle1
Oliver Stegle1
D J Gaffney1
1Wellcome Sanger Institute
Ami Day

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/20f37aaf-caa1-40e6-9123-be6ce8feb2d6
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INSDC Project Accessions:INSDC Study Accessions:

Atlas

None

Analysis Portals

None

Project Label

DopaminergicNeuronDifferentiation

Species

Homo sapiens

Sample Type

cellLines

Anatomical Entity

skin of body

Organ Part

Unspecified

Selected Cell Types

neuron

Model Organ

brain

Disease Status (Specimen)

normal

Disease Status (Donor)

normal

Development Stage

human adult stage

Library Construction Method

10x 3' v2

Nucleic Acid Source

single cell

Paired End

false

File Format

fastq.gz

Cell Count Estimate

1.0M

Donor Count

128
fastq.gz968 file(s)