HCA Data Explorer

Single-cell transcriptomics uncovers distinct molecular signatures of stem cells in chronic myeloid leukemia

Updated September 18, 2023

Single-cell whole transcriptome analysis of chronic myeloid leukemia stem cells, defined as Lin-CD34+CD38-.

Supat ThongjueaWeatheral Institute of Molecular Medicinesupat.thongjuea@imm.ox.ac.uk
Supat Thongjuea (Computational Scientist)1
1Weatheral Institute of Molecular Medicine
Jessica Uhlenhop
Beagan Nguy
Schwartz Rachel

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/2a72a4e5-66b2-405a-bb7c-1e463e8febb0

Supplementary links are provided by contributors and represent items such as additional data which can’t be hosted here; code that was used to analyze this data; or tools and visualizations associated with this specific dataset.

1.ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE76nnn/GSE76312/suppl/GSE76312_Giustacchini-Thongjuea_et.al_Cell_Annotation.txt.gz,ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE76nnn/GSE76312/suppl/GSE76312_Giustacchini_Thongjuea_et.al_Nat.Med.RPKM.txt.gz,ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE76nnn/GSE76312/suppl/GSE76312_Single_cell_HSCs_expression_RPKM_table.rdata.gz
INSDC Project Accessions:
SRP067759
GEO Series Accessions:
GSE76312

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Analysis Portals

None

Project Label

StemCellsInChronicMyeloidLeukemia

Species

Homo sapiens

Sample Type

2 sample types

Anatomical Entity

hematopoietic system

Organ Part

bone marrow

Selected Cell Types

CD34-positive, CD38-negative hematopoietic stem cell

Model Organ

hematopoietic system

Disease Status (Specimen)

2 disease statuses

Disease Status (Donor)

2 disease statuses

Development Stage

human adult stage

Library Construction Method

Smart-seq2

Nucleic Acid Source

single cell

Paired End

false

Analysis Protocol

MultiSampleSmartSeq2_v2.2.6, SmartSeq2SingleSample_v5.1.5

File Format

6 file formats

Cell Count Estimate

2.3k

Donor Count

42
bai2,289 file(s)bam2,289 file(s)fastq.gz2,289 file(s)loom1 file(s)txt.gz2 file(s)xlsx1 file(s)