Single-cell transcriptomics uncovers distinct molecular signatures of stem cells in chronic myeloid leukemia

Access Granted
Updated February 14, 2025

Single-cell whole transcriptome analysis of chronic myeloid leukemia stem cells, defined as Lin-CD34+CD38-.

Supat ThongjueaWeatheral Institute of Molecular Medicinesupat.thongjuea@imm.ox.ac.uk
Supat Thongjuea (Computational Scientist)1
1Weatheral Institute of Molecular Medicine
Jessica Uhlenhop
Beagan Nguy
Schwartz Rachel

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/2a72a4e5-66b2-405a-bb7c-1e463e8febb0

Supplementary links are provided by contributors and represent items such as additional data which can’t be hosted here; code that was used to analyze this data; or tools and visualizations associated with this specific dataset.

1.ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE76nnn/GSE76312/suppl/GSE76312_Giustacchini-Thongjuea_et.al_Cell_Annotation.txt.gz,ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE76nnn/GSE76312/suppl/GSE76312_Giustacchini_Thongjuea_et.al_Nat.Med.RPKM.txt.gz,ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE76nnn/GSE76312/suppl/GSE76312_Single_cell_HSCs_expression_RPKM_table.rdata.gz
INSDC Project Accessions:
SRP067759
GEO Series Accessions:
GSE76312

Downloaded and exported data is governed by the HCA Data Release Policy and licensed under the Creative Commons Attribution 4.0 International License (CC BY 4.0). For more information please see our Data Use Agreement.

Atlas

None

Analysis Portals

None

Project Label

StemCellsInChronicMyeloidLeukemia

Species

Homo sapiens

Sample Type

2 sample types

Anatomical Entity

hematopoietic system

Organ Part

bone marrow

Selected Cell Types

CD34-positive, CD38-negative hematopoietic stem cell

Model Organ

hematopoietic system

Disease Status (Specimen)

2 disease statuses

Disease Status (Donor)

2 disease statuses

Development Stage

human adult stage

Library Construction Method

Smart-seq2

Nucleic Acid Source

single cell

Paired End

false

Analysis Protocol

MultiSampleSmartSeq2_v2.2.6, SmartSeq2SingleSample_v5.1.5

File Format

6 file formats

Cell Count Estimate

2.3k

Donor Count

42
bai2,289 file(s)bam2,289 file(s)fastq.gz2,289 file(s)loom1 file(s)txt.gz2 file(s)xlsx1 file(s)