HCA Data Explorer

GM-CSF-producing T helper cells: a distinct subset of T helper cells.

Access Granted
Updated January 10, 2024

Single-cell RNA sequencing of memory T cells (CD45RA-CD4+) isolated from the peripheral blood (PBMCs) of 5 healthy human donors. Memory T cells were activated/stimulated with PMA/Ion/GP. A population of GM-CSF-producing Th cells (ThGM) was identified that lacked expression of signature transcription factors and cytokines of established Th lineages.

James GarifallouChildren's Hospital of Philadelphiagarifalloj@email.chop.edu
Javad Rasouli1
Giacomo Casella1
Satoshi Yoshimura1
Weifeng Zhang1
Dan Xiao1
James Garifallou2
Michael Gonzalez2
Alice Wiedeman3
Elisabeth R Mari1
Hakon Hakonarson2
S A Long3
Guang-Xian Zhang1
Bogoljub Ciric1
Abdolmohamad Rostami1
1Thomas Jefferson University, Philadelphia
2Children's Hospital of Philadelphia
3Benaroya Research Institute at Virginia Mason
Ami Day

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/41fb1734-a121-4616-95c7-3b732c9433c7
None
INSDC Project Accessions:GEO Series Accessions:INSDC Study Accessions:

Atlas

None

Analysis Portals

None

Project Label

TCellsNeuroinflammation

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

blood

Organ Part

Unspecified

Selected Cell Types

memory T cell

Disease Status (Specimen)

normal

Disease Status (Donor)

normal

Development Stage

human adult stage

Library Construction Method

10x 3' v3 sequencing

Nucleic Acid Source

single cell

Paired End

false

File Format

5 file formats

Cell Count Estimate

70.0k

Donor Count

5
bam5 file(s)fastq.gz30 file(s)loom6 file(s)tar1 file(s)xlsx1 file(s)