HCA Data Explorer

A comprehensive single cell transcriptional landscape of human hematopoietic progenitors

Access Granted
Updated March 10, 2022

Hematopoietic Stem/Progenitor cells (HSPCs) are endowed with the role of maintaining a diverse pool of blood cells throughout the human life. Despite recent efforts, the nature of the early cell fate decisions remains contentious. Using single-cell RNA-Seq, we show that existing approaches to stratify bone marrow CD34+ cells reveal a hierarchically-structured transcriptional landscape of hematopoietic differentiation. Still, this landscape misses important early fate decisions. We here provide a broader transcriptional profiling of bone marrow lineage negative hematopoietic progenitors that recovers a key missing branchpoint into basophils and expands our understanding of the underlying structure of early adult human haematopoiesis. We also show that this map has strong similarities in topology and gene expression to that found in mouse. Finally, we identify the sialomucin CD164, as a reliable marker for the earliest branches of HSPCs specification and we showed how its use can foster the design of alternative transplantation cell products.

Luca BiascoDana-Farber, Boston Children’s Cancer and Blood Disorders Centerl.biasco@ucl.ac.uk
Luca Biasco (Principal Investigator)1
1Dana-Farber, Boston Children’s Cancer and Blood Disorders Center
Parisa Nejad

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/5116c081-8be7-49c5-8ce0-73b887328aa9

Supplementary links are provided by contributors and represent items such as additional data which can’t be hosted here; code that was used to analyze this data; or tools and visualizations associated with this specific dataset.

1.ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE117nnn/GSE117498/suppl/GSE117498_RAW.tar
INSDC Project Accessions:GEO Series Accessions:

Analysis Portals

None

Project Label

HumanHematopoieticProgenitors

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

hematopoietic system

Organ Part

bone marrow

Selected Cell Types

8 cell types

Disease Status (Specimen)

normal

Disease Status (Donor)

normal

Development Stage

human adult stage

Library Construction Method

inDrop

Nucleic Acid Source

single cell

Paired End

false

Analysis Protocol

analysis_protocol_1

File Format

3 file formats

Cell Count Estimate

Unspecified

Donor Count

2
fastq.gz60 file(s)tsv.gz11 file(s)xlsx1 file(s)