HCA Data Explorer

Single-Cell Transcriptomic Profiling of Human Dental Pulp in Sound and Carious Teeth: A Pilot Study

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Updated January 26, 2023

We present a collection of single-cell transcriptomic profiles of 6,810 pulpal cells isolated from a sound human maxillary third molars and carious teeth at different stages. We showed that the presence of deep, but not enamel caries, altered the immune cell compositions of the dental pulp. Differential expression analysis further revealed that the pro-inflammatory, anti-inflammatory and mineralization-related genes were upregulated in immune and stromal cells in deep dental caries. Cell-cell interaction prediction showed potential interactions between immune and stromal cells during homeostasis, and enhanced interactions between different cell types with macrophage during deep dental caries. Taken together, our study serves as a comprehensive survey of human pulpal cell heterogeneity, as well as provides novel molecular insights into dental pulps in health and disease. Overall design: The single-cell transcriptomic profiles from cells residing in dental pulp from a sound tooth, enamel caries and deep dental caries were compared. Cell type frequency and differential gene expression were analyzed. Cell-cell interactions were predicted from the expression level of ligands and receptors.

Anunya OpasawatchaiMahidol Universityanunya.opa@mahidol.edu
Varodom CharoensawanMahidol Universityvarodom.cha@mahidol.ac.th
Anunya Opasawatchai (Experimental Scientist)1
Varodom Charoensawan (Experimental Scientist)1
1Mahidol University
Parisa Nejad
Brittney D Wick
Rachel Schwartz

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/6f89a7f3-8d4a-4344-aa4f-eccfe7e91076
None
INSDC Project Accessions:GEO Series Accessions:INSDC Study Accessions:

Atlas

None

Analysis Portals

None

Project Label

humanDentalPulpCells

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

2 anatomical entities

Organ Part

dental pulp

Selected Cell Types

Unspecified

Disease Status (Specimen)

3 disease statuses

Disease Status (Donor)

3 disease statuses

Development Stage

human adult stage

Library Construction Method

10x 3' v2

Nucleic Acid Source

single cell

Paired End

false

Analysis Protocol

analysis_protocol_1

File Format

3 file formats

Cell Count Estimate

6.8k

Donor Count

4
csv.gz4 file(s)fastq.gz14 file(s)xlsx1 file(s)