HCA Data Explorer

The Human and Mouse Enteric Nervous System at Single-Cell Resolution.

Access Granted
Updated July 22, 2022

The authors develop two methods to profile the Enteric Nervous System of adult mice and humans at single-cell resolution: RAISIN RNA-seq for profiling intact nuclei with ribosome-bound mRNA and MIRACL-seq for label-free enrichment of rare cell types by droplet-based profiling.

Ramnik J XavierBroad Institute of MIT and Harvardxavier@molbio.mgh.harvard.edu
Aviv RegevGenentecharegev@broadinstitute.org
Eugene Drokhlyansky1
Christopher S Smillie1
Nicholas Van Wittenberghe1
Maria Ericsson2
Gabriel K Griffin1
Gokcen Eraslan1
Danielle Dionne1
Michael S Cuoco1
Max N Goder-Reiser3
Tatyana Sharova3
Olena Kuksenko1
Andrew J Aguirre4
Genevieve M Boland3
Daniel Graham4
Orit Rozenblatt-Rosen1
Ramnik J Xavier4
Aviv Regev5
1Klarman Cell Observatory, Broad Institute of MIT and Harvard
2Harvard Medical School
3Massachusetts General Hospital
4Broad Institute of MIT and Harvard
5Genentech
Ami Day

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/7be05025-9972-493a-856f-3342a8d1b183

Supplementary links are provided by contributors and represent items such as additional data which can’t be hosted here; code that was used to analyze this data; or tools and visualizations associated with this specific dataset.

1.https://duos.broadinstitute.org/dataset_catalog2.https://github.com/klarman-cell-observatory/ens_atlas3.https://singlecell.broadinstitute.org/single_cell/study/SCP1038/the-human-and-mouse-enteric-nervous-system-at-single-cell-resolution
None

Atlas

None

Analysis Portals

None

Project Label

HumanMouseEntericNervousSystem

Species

2 species

Sample Type

specimens

Anatomical Entity

2 anatomical entities

Organ Part

3 organ parts

Selected Cell Types

Unspecified

Disease Status (Specimen)

normal

Disease Status (Donor)

normal

Development Stage

2 development stages

Library Construction Method

2 library construction methods

Nucleic Acid Source

2 nucleic acid sources

Paired End

false, true

Analysis Protocol

analysis_protocol_cell_type_assignment, analysis_protocol_matrix_generation

File Format

4 file formats

Cell Count Estimate

1.6M

Donor Count

52
fastq.gz171 file(s)mtx5 file(s)tsv10 file(s)txt1 file(s)