HCA Data Explorer

Comparison of manual and two types of bioprinted kidney organoids by single cell RNA-seq.

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Updated August 31, 2022

4 independent pools of iPS cells were differentiated and used to generate kidney organoids. Each pool was used to generate each of the 3 types of conformations: Manual organoids, bioprinted organoids composed of a single deposition of cells (R0) and bioprinted organoids composed a thin line of cells (R40). Manual and two types of bioprinted kidney organoids were analysed by single cell RNA-seq.

Kynan T LawlorMurdoch Children's Research Institutekynan.lawlor@mcri.edu.au
Melissa H LittleMurdoch Children's Research Institutemelissa.little@mcri.edu.au
Kynan T Lawlor1
Jessica M Vanslambrouck1
J W Higgins2
Alison Chambon2
Kristina Bishard2
Derek Arndt2
Pei Xuan Er1
Sean B Wilson1
Sara E Howden1
Ker Sin Tan1
Fanyi Li1
Lorna J Hale 1
Benjamin Shepherd2
Stephen Pentoney2
Sharon C Presnell 2
Alice E Chen2
Melissa H Little1
1Murdoch Children's Research Institute
2Organovo
Ami Day

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/8787c238-89ef-4636-a57d-3167e8b54a80
None
INSDC Project Accessions:GEO Series Accessions:INSDC Study Accessions:

Atlas

None

Analysis Portals

None

Project Label

KidneyOrganoidsBioprinting

Species

Homo sapiens

Sample Type

organoids

Anatomical Entity

skin of prepuce of penis

Organ Part

Unspecified

Selected Cell Types

Unspecified

Model Organ

kidney

Disease Status (Specimen)

normal

Disease Status (Donor)

normal

Development Stage

human adult stage

Library Construction Method

10x 3' v3

Nucleic Acid Source

single cell

Paired End

false

File Format

fastq.gz

Cell Count Estimate

106.0k

Donor Count

1
fastq.gz60 file(s)