A Human Liver Cell Atlas reveals Heterogeneity and Epithelial Progenitors
We perfomed single-cell RNA-sequnecing of around 10,000 cells from normal human liver tissue to construct a human liver cell atlas. We reveal previously unknown subtypes in different cell type compartments. Overall design: Single cells were isolated from human liver resection specimens and then sorted by FACS into 384 well plates in a unbiased way and on the basis of cell surface markers for distinct cell types. ScRNA-seq was done using the mCelSeq2 protocol.
A human liver cell atlas reveals heterogeneity and epithelial progenitors. (Official HCA Publication)
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Atlas
Analysis Portals
NoneProject Label
LiverCellAtlasHeterogeneitySpecies
Homo sapiens
Sample Type
Anatomical Entity
liver
Organ Part
Unspecified
Selected Cell Types
Unspecified
Model Organ
liver
Disease Status (Specimen)
Disease Status (Donor)
Development Stage
human adult stage
Library Construction Method
CEL-seq2
Nucleic Acid Source
single cell
Paired End
falseAnalysis Protocol
analysis_protocol_normalization, analysis_protocol_quantificationFile Format
Cell Count Estimate
10.0kDonor Count
23