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Assessing Cellular and Transcriptional Diversity of Ileal Mucosa Among Treatment-Naïve and Treated Crohn’s Disease

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Updated December 9, 2024

Crohn’s disease (CD) is a chronic inflammatory disease of the gastrointestinal tract. We used single cell RNA sequencing (scRNAseq) to define the cellular and transcriptional activity of the mucosa at different stages of disease progression in CD. Overall design: Terminal ileal mucosal derived single cells was processed and analyzed using scRNAseq

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Assessing Cellular and Transcriptional Diversity of Ileal Mucosa Among Treatment-Naïve and Treated Crohn's Disease

Sushma C Maddipatla1
Vasantha L Kolachala1
Suresh Venkateswaran (Experimental Scientist)1
Anne F Dodd1
Ranjit S Pelia1
Duke Geem1
Hong Yin1
Yutong Sun2
Congmin Xu2
Angela Mo2
Astrid Kosters1
Junkai Yang1
Jason D Matthews1
Eliver Ghosn1
Subra Kugathasan1
Peng Qiu2
1Emory University School of Medicine
2Georgia Institute of Technology
Bhavik Chand

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/9dd91b6e-7c62-49d3-a3d4-74f603deffdb
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GEO Series Accessions:INSDC Project Accessions:INSDC Study Accessions:

Atlas

GutGut v1.0

Analysis Portals

None

Project Label

diversityOfIlealMucosa

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

ileum

Organ Part

ileal mucosa

Selected Cell Types

Unspecified

Disease Status (Specimen)

2 disease statuses

Disease Status (Donor)

2 disease statuses

Development Stage

3 development stages

Library Construction Method

10x 5' v1

Nucleic Acid Source

single cell

Paired End

true

Analysis Protocol

analysis_protocol_1

File Format

4 file formats

Cell Count Estimate

139.9k

Donor Count

27
fastq.gz133 file(s)mtx.gz27 file(s)tsv.gz54 file(s)xlsx1 file(s)
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