Single cell RNA-seq of brain glioblastoma samples
Updated January 26, 2023Glioblastomas (GBM) are the most aggressive type of brain tumors with a dismal prognosis. The effectiveness of anti-tumor drugs is thwarted by brain endothelial cells (ECs) in the tumor microenvironment. However, the characteristics of ECs remains poorly inventoried at the single-cell level. We performed single-cell RNA sequencing (scRNA-seq) for ECs from 4 GBMs and matching non-malignant brain samples, and identified distinct EC phenotypes in GBM. Overall design: We profiled freshly isolated cells from tumor core and paired surrounding peripheral tissue in 4 glioblastoma patients. Single-cell suspensions, magnetic-activated cell sorting (MACS) enriched for CD31+ cells, were subjected to scRNA-seq using a 10X genomics-based single-cell protocol.
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Analysis Portals
NoneProject Label
HumanBrainEndothelialGBMSpecies
Sample Type
Anatomical Entity
Organ Part
Selected Cell Types
Disease Status (Specimen)
Disease Status (Donor)
Development Stage
Library Construction Method
Nucleic Acid Source
Paired End
falseAnalysis Protocol
analysis_protocol_1File Format
Cell Count Estimate
UnspecifiedDonor Count
4