A cell atlas of human thymic development defines T cell repertoire formation
We performed single-cell RNA-sequencing to create a cell census of the human thymus during development, early childhood and adult life. We sampled 15 embryonic and fetal thymi spanning thymic developmental stages between 7 post-conception weeks (PCW) to 17 PCW, and 9 postnatal thymi from paediatric and adult samples. We compared the cellular composition and T cell differentiation within human and mouse thymus using newly generated and repository mouse datasets. Finally, we investigated the bias in the recombination and selection of human versus mouse TCR repertoires.
A cell atlas of human thymic development defines T cell repertoire formation. (Official HCA Publication)
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Analysis Portals
Project Label
HumanThymicDevelopmentSpecies
Sample Type
specimens
Anatomical Entity
Organ Part
Unspecified
Selected Cell Types
Disease Status (Specimen)
normal
Disease Status (Donor)
normal
Development Stage
Library Construction Method
Nucleic Acid Source
single cell
Paired End
falseAnalysis Protocol
optimus_post_processing_v1.0.0, optimus_v4.2.3File Format
Cell Count Estimate
250.0kDonor Count
24