Single-cell omics reveal human mononuclear phagocyte heterogeneity and inflammatory DC in health and disease
Human mononuclear phagocytes comprise phenotypically and functionally overlapping subsets of dendritic cells (DC) and monocytes, but their identification remains elusive. By integrating high-dimensional single-cell protein and RNA expression data, we clearly delineated monocytes from conventional DC2 (cDC2), identifying new markers including CD88/CD89 for monocytes and HLA-DQ/FcRI for cDC2, allowing their unambiguous characterization in blood and tissues. We also show that cDC2 can be subdivided into phenotypically and functionally distinct subsets based on CD5, CD163 and CD14 expression, including a unique subset of circulating inflammatory CD5-CD163+CD14+ cells related to previously defined DC3. These inflammatory DC3 were expanded in systemic lupus erythematosus patients, correlating with disease activity. Unravelling the heterogeneity of DC sub-populations in health and disease paves the way for specific DC subset-targeting therapies.
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Analysis Portals
NoneProject Label
HumanPhagocytesHealthyLupusDutetreSpecies
Homo sapiens
Sample Type
specimens
Anatomical Entity
blood
Organ Part
Unspecified
Selected Cell Types
Disease Status (Specimen)
normal
Disease Status (Donor)
Unspecified
Development Stage
human adult stage
Library Construction Method
Smart-seq2
Nucleic Acid Source
single cell
Paired End
trueAnalysis Protocol
MultiSampleSmartSeq2_v2.2.6, SmartSeq2SingleSample_v5.1.5File Format
Cell Count Estimate
221Donor Count
1