HCA Data Explorer

Single-cell omics reveal human mononuclear phagocyte heterogeneity and inflammatory DC in health and disease

Access Granted
Updated January 19, 2022

Human mononuclear phagocytes comprise phenotypically and functionally overlapping subsets of dendritic cells (DC) and monocytes, but their identification remains elusive. By integrating high-dimensional single-cell protein and RNA expression data, we clearly delineated monocytes from conventional DC2 (cDC2), identifying new markers including CD88/CD89 for monocytes and HLA-DQ/FcRI for cDC2, allowing their unambiguous characterization in blood and tissues. We also show that cDC2 can be subdivided into phenotypically and functionally distinct subsets based on CD5, CD163 and CD14 expression, including a unique subset of circulating inflammatory CD5-CD163+CD14+ cells related to previously defined DC3. These inflammatory DC3 were expanded in systemic lupus erythematosus patients, correlating with disease activity. Unravelling the heterogeneity of DC sub-populations in health and disease paves the way for specific DC subset-targeting therapies.

Florent GinhouxSingapore Immunology Network (SIgN), A*STARFlorent_Ginhoux@immunol.a-star.edu.sg
Florent Ginhoux (Experimental Scientist)1
1Singapore Immunology Network (SIgN), A*STAR
Teresa Marquez

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/ccd1f1ba-74ce-469b-9fc9-f6faea623358

Supplementary links are provided by contributors and represent items such as additional data which can’t be hosted here; code that was used to analyze this data; or tools and visualizations associated with this specific dataset.

1.ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE132nnn/GSE132566/suppl/GSE132566_RAW.tar
INSDC Project Accessions:GEO Series Accessions:INSDC Study Accessions:

Analysis Portals

None

Project Label

HumanPhagocytesHealthyLupusDutetre

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

blood

Organ Part

Unspecified

Selected Cell Types

2 cell types

Disease Status (Specimen)

normal

Disease Status (Donor)

Unspecified

Development Stage

human adult stage

Library Construction Method

Smart-seq2

Nucleic Acid Source

single cell

Paired End

true

Analysis Protocol

MultiSampleSmartSeq2_v2.2.6, SmartSeq2SingleSample_v5.1.5

File Format

5 file formats

Cell Count Estimate

221

Donor Count

1
bai221 file(s)bam221 file(s)fastq.gz442 file(s)loom1 file(s)tar1 file(s)