Single-nucleus RNA sequencing of human cortex affected by multiple sclerosis.

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Updated October 31, 2022

Single-nucleus RNA sequencing of human cortex affected by multiple sclerosis cortical lesions.

Arnold R KriegsteinEli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of Californiaarnold.kriegstein@ucsf.edu
David H RowitchEli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of Californiadhr25@medschl.cam.ac.uk
Lucas Schirmer1
Dmitry Velmeshev1
Staffan Holmqvist2
Max Kaufmann3
Sebastian Werneburg4
Diane Jung1
Stephanie Vistnes1
John H Stockley2
Adam Young2
Maike Steindel2
Brian Tung1
Nitasha Goyal1
Aparna Bhaduri1
Simone Mayer1
Jan Broder Engler3
Omer A Bayraktar2
Robin JM Franklin2
Maximilian Haeussler5
Richard Reynolds6
Dorothy P Schafer4
Manuel A Friese3
Lawrence R Shiow1
Arnold R Kriegstein1
David H Rowitch1
1Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California
2University of Cambridge
3Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf
4University of Massachusetts Medical School
5Genomics Institute, University of California
6Imperial College London
Ami Day

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/ce7b12ba-664f-4f79-8fc7-3de6b1892183
None
INSDC Project Accessions:
INSDC Study Accessions:

Atlas

None

Analysis Portals

UCSC Cell Browser
UCSC Cell Browser

Project Label

MultipleSclerosisLineageDiversity

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

brain

Organ Part

3 organ parts

Selected Cell Types

Unspecified

Disease Status (Specimen)

2 disease statuses

Disease Status (Donor)

2 disease statuses

Development Stage

human adult stage

Library Construction Method

10X 3' v2 sequencing

Nucleic Acid Source

single nucleus

Paired End

false

Analysis Protocol

analysis_protocol, optimus_post_processing_v1.0.0, optimus_v4.2.3

File Format

5 file formats

Cell Count Estimate

84.0k

Donor Count

21
bam21 file(s)fastq.gz42 file(s)loom22 file(s)tar1 file(s)xlsx2 file(s)
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