HCA Data Explorer

Single-cell RNA-seq of bone marrow cells from aplastic anemia patient and healthy donor

Access Granted
Updated August 30, 2022

BM samples were collected from two adult healthy donors and two AA patients at the first hospital affiliated from Zhejiang Chinese University. Mononuclear cells (MNCs) were isolated using Ficoll-Hypaque gradient separation. CD34+ cells were purified from MNCs with the human anti-CD34 MicroBeads Isolation kit according to the manufactures specifications. Isolated cell was the purification of CD34+ cell. Then, MNCs mixed with CD34+ cells at 4:1 ratio and were analyzed by 10×Genomics. Overall design: a large-scale single-cell transcriptomic sequencing of 20,000 bone marrow cells from two adult healthy donors and two AA patients

Yin LimingFirst Affiliated Hospital of Zhejiang Chinese Medical Universityyinlm0509@zcmu.edu.cn
Yin Liming (Principal Investigator)1
1First Affiliated Hospital of Zhejiang Chinese Medical University
Rachel Schwartz

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/e993adcd-d4ba-4f88-9a05-d1c05bdf0c45
None
GEO Series Accessions:INSDC Project Accessions:INSDC Study Accessions:

Analysis Portals

None

Project Label

boneMarrowAnemia

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

hematopoietic system

Organ Part

bone marrow

Selected Cell Types

2 cell types

Disease Status (Specimen)

2 disease statuses

Disease Status (Donor)

2 disease statuses

Development Stage

human adult stage

Library Construction Method

2 library construction methods

Nucleic Acid Source

single cell

Paired End

false

Analysis Protocol

analysis_protocol_1, analysis_protocol_2

File Format

4 file formats

Cell Count Estimate

20.0k

Donor Count

4
fastq.gz40 file(s)tar1 file(s)txt.gz1 file(s)xlsx1 file(s)