Single-cell RNA-seq of bone marrow cells from aplastic anemia patient and healthy donor
BM samples were collected from two adult healthy donors and two AA patients at the first hospital affiliated from Zhejiang Chinese University. Mononuclear cells (MNCs) were isolated using Ficoll-Hypaque gradient separation. CD34+ cells were purified from MNCs with the human anti-CD34 MicroBeads Isolation kit according to the manufactures specifications. Isolated cell was the purification of CD34+ cell. Then, MNCs mixed with CD34+ cells at 4:1 ratio and were analyzed by 10×Genomics. Overall design: a large-scale single-cell transcriptomic sequencing of 20,000 bone marrow cells from two adult healthy donors and two AA patients
To reference this project, please use the following link:
Downloaded and exported data is governed by the HCA Data Release Policy and licensed under the Creative Commons Attribution 4.0 International License (CC BY 4.0). For more information please see our Data Use Agreement.
Analysis Portals
NoneProject Label
boneMarrowAnemiaSpecies
Homo sapiens
Sample Type
specimens
Anatomical Entity
hematopoietic system
Organ Part
bone marrow
Selected Cell Types
Disease Status (Specimen)
Disease Status (Donor)
Development Stage
human adult stage
Library Construction Method
Nucleic Acid Source
single cell
Paired End
falseAnalysis Protocol
analysis_protocol_1, analysis_protocol_2File Format
Cell Count Estimate
20.0kDonor Count
4