Dissecting the protein and transcriptional responses of human immune cells to T cell and monocyte specific activation
Single cell profiling is a powerful tool for studying the molecular and cellular (dys)function of activated peripheral blood mononuclear cells (PBMCs) in the context of disease. We combined CITE-seq with two levels of multiplexing (cell hashing and individuals’ genotypes) to derive a reference database of immune cell gene/protein responses to different activation conditions. PBMCs from 10 healthy adults were profiled before and after stimulating i) T cells via anti-CD3/CD28 or ii) monocytes via LPS. By using a comprehensive antibody panel (n=39) of cell type (e.g., CD16, CD14) and cell state (e.g., CD69, CD25) markers, we discovered that all lymphocytes responded to anti-CD3/CD28 stimulation, whereas LPS specifically induced inflammation in monocytes. Pseudo-temporal analyses further revealed cell- and condition-specific heterogeneity in responses to activation that were independent of individual-specific variation. Together, these data are shared within an interactive web application (https://czi-pbmc-cite-seq.jax.org/) and will serve as a resource to guide future studies of immune cell responses.
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Analysis Portals
NoneProject Label
CITEseqPBMCProjectSpecies
Homo sapiens
Sample Type
specimens
Anatomical Entity
blood
Organ Part
Unspecified
Selected Cell Types
Unspecified
Disease Status (Specimen)
Unspecified
Disease Status (Donor)
Unspecified
Development Stage
human adult stage
Library Construction Method
CITE-seq
Nucleic Acid Source
single cell
Paired End
falseFile Format
Cell Count Estimate
250.0kDonor Count
10